生长抑素受体2的小鼠时空组织表达谱

生长抑素(somatostatin, SST)作为一种抑制性多肽激素,在多种生物过程中发挥重要的功能。生长抑素受体2 (somatostatin receptor 2, SSTR2)作为生长抑素表达最广泛的受体在多种组织中表达,但其表达的具体细胞类型尚不清楚。本研究在小鼠不同发育阶段的多种组织中鉴定了SSTR2蛋白表达的细胞类型。通过多色免疫荧光在小鼠胚胎期15.5 d、出生后1 d、7 d、15 d、3个月和6个月的脑、骨、肺、肠道、皮肤、胃、脾和肾等组织中检测了Sstr2基因的表达。结果发现Sstr2在不同发育阶段的多种组织的特定细胞类型中表达,包括脑神经元和星形胶质细胞,骨的间充质基质细胞、造血细胞和B细胞,肺的巨噬细胞、Ⅱ型肺泡上皮细胞和气道纤毛细胞,肠道的上皮细胞和神经元,皮肤的毛囊细胞,胃体的上皮细胞,脾的造血干细胞、造血祖细胞和神经纤维,肾的肾小管上皮细胞等。本研究确定了小鼠多组织不同发育阶段Sstr2表达的细胞类型,为生长抑素与生长抑素受体2的生理功能研究提供了新的线索。     

转基因玉米HGK60在不同遗传背景下抗虫性鉴定及农艺性状分析

为研究转基因玉米HGK60在不同遗传背景下遗传稳定性和抗虫效果,利用转Bt cry1Ah基因的转基因玉米HGK60为供体,通过回交转育的方式将cry1Ah基因分别导入玉米自交系郑58、昌7-2、lx05-4、lx03-2,获得转基因玉米自交系HGK60-郑58、HGK60-昌7-2、HGK60-lx03-2、HGK60-lx05-4,并杂交获得HGK60-郑单958（HGK60-郑58 × HGK60-昌7-2）和HGK60-鲁单9066（HGK60-lx05-4 × HGK60-lx03-2）,转化体特异性PCR证明cry1Ah基因已转入不同遗传背景玉米中,ELISA检测不同遗传背景转基因玉米叶片中Cry1Ah蛋白表达情况,结果表明在不同遗传背景玉米自交系和杂交种中Cry1Ah蛋白表达没有显著差异;田间人工接虫和室内玉米螟抗虫性鉴定结果表明,不同遗传背景的转基因玉米高抗玉米螟,室内接虫后4 d幼虫死亡率达到100%;对不同遗传背景转基因玉米HGK60进行农艺性状分析,结果显示与受体对照玉米相比,两者之间农艺性状没有显著差异,转基因玉米HGK60可用于抗虫玉米品种的选育。     

BIOACTIVITIES AND MECHANISM OF SPIRO ENOL ETHER ANALOGUES AGAINST PIERIS RAPAE

Abstract  Nineteen kinds of spiro enol ether analogues were screened with larvae of Pieris rapae for antifeedant activity. The results showed that the antifeedant activity of compounds No.20 and No. 12 was higher than others. In non-choice test, AFC50 values within 24 h of compounds No.20 and No. 12 against 3rd instar larvae of P. rapae were 226.93 μg/mL and 370.00 μg/mL, and that in choice test against 4th larvae were 280.54 μg/mL and 398.88 μg/mL, respectively. Compd. No.20 could prolong the eggs hatch time and reduce the haemolymph content and the protein content in haemolymph of 4th instar larvae obviously. Compd. No.20 could protect tested leaves and control larvae of P. rapae effectively.     

革螨跗感器的结构和功能

本文进一步研究了:④厩真厉螨截肢前后的爬行行为,表明第1对足其感觉功能;②用古拉广厉螨分别截各对足驱避反应的对比试验,见到只有当截去第1对足跗节时失去嗅觉功能,而截去第Ⅱ、Ⅲ、Ⅳ对足时,各组都仍有嗅觉功能;⑤对格氏血厉螨、厩真厉螨、毒厉螨和鼠颚毛厉螨进行涂漆前后的驱避试验,显示当跗感器被涂满封闭,则嗅觉功能消失;④以0.5%结晶紫或龙胆紫液染色的截肢标本,观察了厩真厉螨、毒厉螨、格氏血厉螨、古拉广厉螨及尾足螨股一种螨,足Ⅰ跗节末端凹窝中,至少都存在两类感毛,钝钉型感毛和长而尖的刚毛型感毛;⑤厩真厉螨雌螨和幼螨跗感器的钝钉毛分别为8根和5根,另外各有2根短而尖的毛,分别测定了长度,描述了形态特点;⑥透射电镜观察厩真厉螨、毒厉螨等的跗感器钝钉毛,毛外围有表皮壁,壁上有很多微孔,内有中心腔,腔内有树突。属化感器——嗅觉器;⑦电生理技术研究,当用氨和醋酸的气体刺激厩真厉螨、毒厉螨的离体足Ⅰ时,均产生明显的应激电位差,充分证明足Ⅰ辩节有嗅觉功能。     

Spatial and Temporal Variability during Periods of "Recovery" after Mass Bleaching on Western Atlantic Coral Reefs

Western Atlantic coral reefs were differentially affected bya mass bleaching (discoloration) event in 1987. We periodicallyassessed the "appearance" of zooxanthellate organisms betweenDecember 1987 and June 1988 at nine conspicuously affected sitesin the Bahamas, Florida, St. Croix, and Venezuela, using a standardizedpoint-count technique. Three to four months after the localinitiation of the event, the "bleached" state was still presentin one to three of the most abundant reef coral taxa and ina few of the less common species (n = 5 sites). "Recovery" occurredsomewhat faster at shallower depths, at least in the Bahamasand Florida. Scleractinian corals which were "prolonged bleachers"had foliaceous or massive, rather than branching, morphologies."Bleached" points disappeared from the point counts after $6to $8 months. Long-term field data on spatial and temporal variability inthe dynamics of zooxanthellate organisms would help us to understandthe ecological consequences of bleaching. More generally, weneed to distinguish anthropogenic changes in the structure andfunctioning of reef ecosystems from those which occur naturally.Point-count techniques are well suited for collaborative studiesinvolving rapid quantification of coloration states and healthin reef corals.     

杭州双睾虫生活史各阶段体壁的透射电镜观察

作者应用透射电镜技术观察了杭州双睾虫(Diplorchis hangzhouensis)生活史各阶段(自由生活的钩毛蚴、蝌蚪鳃上的后期幼虫、沼蛙膀胱内的成虫)体壁的超微结构,讨论了幼虫发育至成虫过程中这些结构演变的意义,并与其它单殖吸虫的体壁进行了比较。